Improved Editing through CRISPR-targeted Transposable Element InsertionMarch 22, 2023
Researchers from Donald Danforth Plant Science Center and partners developed a genome editing tool that controls the transposable elements' insertion site and cargo delivery. Their preliminary findings are published in Research Square.
The present tools used to insert DNA in specific sites in the plant genome are low-frequency and error-prone. Transposable elements, which are considered genome ‘parasites' have evolved to insert their DNA effectively into genomes. They choose the insertion site based on preferences of chromatic contexts, which vary among TE classes. Thus, took advantage of the TE's natural ability to insert the genome precisely and came up with a genome editing tool that controls TE insertion.
The researchers patterned their approach with CRISPR-associated transposases (CASTs) that target transposition in a programmable manner in bacteria. A synthetic CAST was developed by fusing the rice Pong TE transposase protein to the Cas9 or CPF1 programmable nucleases. This led to sequence-specific targeted of enhancer elements, an open reading frame, and a gene expression cassette into the genome of Arabidopsis. The system was also successfully translated into soybeans.
Read more from Research Square.
You might also like:
- Plant Breeding Innovation: CRISPR-Cas9
- Successful Deletion of Rice Retrotransposon Using CRISPR-Cas9
- New Genome of Chinese Soybean Now Available
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
Research and Tools
- Startup Develops Better-tasting Yellow Peas Using CRISPR
- Gene Editing Technology to Fight Corn Disease
- Growing Crops on Mars Possible With Gene Editing
- Improved Editing through CRISPR-targeted Transposable Element Insertion
- Scientists Disable Protective Gene In Mosquitoes Using CRISPR-Cas9
- Gene Knockout Produces “Gentle” Mackerel
Read the latest:
- Biotech Updates (December 7, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: